Induction and assessment of colchicine induced variability in karonda https://doi.org/10.21276/AATCCReview.2025.13.04.648 Krishna https://orcid.org/0009-0000-4179-6437 V. P. Singh https://orcid.org/0009-0000-5924-9060 S. K. Verma https://orcid.org/0000-0002-6738-5334 Ajay Kumar https://orcid.org/0009-0005-0292-1748 Ratna Rai https://orcid.org/0000-0001-8944-7092 Rajesh Kumar https://orcid.org/0009-0007-5137-3463 Satish Chand https://orcid.org Viveka Nand https://orcid.org/0000-0003-3353-268X Virendra Kumar https://orcid.org/0009-0001-8786-659X Karonda (Carissa carandas L.) is a native fruit known for its nutrition, medicinal benefits and diversified uses. Despite its multiple uses, it exhibits a narrow genetic base. Since genetic variability is a prerequisite for any effective crop improvement programme, the present study was conducted to induce variation in this crop. Seeds of Karonda cv. Pant Manohar was treated with different doses of colchicine, ranging from 0.1%-0.9% along with a control. The experiment was conducted using a randomised block design (RBD), with each treatment replicated thrice. The main objective of the current study was to assess the ability of colchicine to induce variation in karonda. Plants showing some variations were selected for further biochemical analysis to check the effectiveness of colchicine in creating variation. Variants were evaluated against the control ones for leaf chlorophyll content, total carotenoid content and relative water content (RWC) in the leaves. The results showed dose-independent variations among the treatments. Whereas, C5 (0.5%) exhibited maximum chlorophyll and carotenoid content, while maximum RWC was recorded in C2 (0.2%). Morphological variations were also observed in treated population. These findings may be further utilized in future crop improvement programmes aimed at variability creation, and genetic improvement in karonda.

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Original Research Article
Krishna 1 V. P. Singh1 S. K. Verma2 Ajay Kumar3 Ratna Rai1 Rajesh Kumar1 Satish Chand1 Viveka Nand4 Virendra Kumar5
1 Department of Horticulture, GB Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, 263145, India
2 Department of Genetics and Plant Breeding, GB Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, 263145, India
3 Department of Agronomy, GB Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, 263145, India
4 Department of Chemistry, GB Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, 263145, India
5 School of Agriculture & Development Studies, Uttarakhand Open University, Haldwani, Uttarakhand (India) , India
https://doi.org/10.21276/AATCCReview.2025.13.04.648

Abstract

Karonda (Carissa carandas L.) is a native fruit known for its nutrition, medicinal benefits and diversified uses. Despite its multiple uses, it exhibits a narrow genetic base. Since genetic variability is a prerequisite for any effective crop improvement programme, the present study was conducted to induce variation in this crop. Seeds of Karonda cv. Pant Manohar was treated with different doses of colchicine, ranging from 0.1%-0.9% along with a control. The experiment was conducted using a randomised block design (RBD), with each treatment replicated thrice. The main objective of the current study was to assess the ability of colchicine to induce variation in karonda. Plants showing some variations were selected for further biochemical analysis to check the effectiveness of colchicine in creating variation. Variants were evaluated against the control ones for leaf chlorophyll content, total carotenoid content and relative water content (RWC) in the leaves. The results showed dose-independent variations among the treatments. Whereas, C5 (0.5%) exhibited maximum chlorophyll and carotenoid content, while maximum RWC was recorded in C2 (0.2%). Morphological variations were also observed in treated population. These findings may be further utilized in future crop improvement programmes aimed at variability creation, and genetic improvement in karonda.

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