An investigation on “Effect of GA3 and Bio-inoculants on vegetative growth of Gladiolus (Gladiolus grandiflorus L.) cv. Nova Lux” was carried out at the Experimental Farm, Division of Floriculture and Landscaping, Sher-e-Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu, during 2022–23 and 2023–24. The factorial randomized block design consists of four levels of GA3 ( 0, 50, 100 and 150 ppm) and five levels of bio-inoculants (No-inoculation, Bacillus subtilis, Actinomycete, Pseudomonas fluorescens and consortia). Overall data shows that among the treatments, T20 consists of GA3 @ 150 ppm combined with consortia (Bacillus subtilis, Actinomycete and Pseudomonas fluorescens) recorded maximum plant height (124.62 cm), number of leaves per plant (9.96), leaf length (63.45 cm), leaf width (5.33 cm) and leaf area (71.28 cm²). The study suggests that the integrated use of GA3 and bio-inoculants can significantly enhance the vegetative growth of gladiolus.