Background: Bispyribac sodium_is the organic sodium salt of bispyribac. It is used as a broad-spectrum post-emergent herbicide for the control of grasses, sedges and broadleaf weeds in rice crops. Diafenthiuron is an aromatic ether that an agricultural pro-insecticide that is used to control mites, aphids, and whitefly in cotton. There is no validated method for quantification of Bispyribac sodium and Difenthiuron in pesticide formulations.
Objective: This study aimed to develop a simple, specific, accurate, precise and reliable UFLC method for the quantification of Bispyribac sodium and Difenthiuron in their pesticide formulations.
Methods: Bispyribac sodium and Difenthiuron were quantified by using Shimadzu, packed with C18 (250 mm × 4.6 mm, 5 μm) column. A mixture of acetonitrile: water (50:50 and 70:30 v/v) was used as a mobile phase for Bispyribac sodium and Difenthiuron respectively. The column oven temperature was maintained at 25°C throughout the analysis. The flow rate was maintained at 1.2 and 1.0 ml/min and detection at 254 and 225 nm for Bispyribac sodium and Difenthiuron respectively was carried out with injection volume of 5 and 2μl. The % RSD values for the precision study were <1.19 and <1.60 for Bispyribac sodium and Difenthiuron respectively. The analytical method was validated according to the guidelines described in Collaborative International Pesticides Analytical Council (CIPAC). Results: The UFLC method was able to quantify Bispyribac sodium and Difenthiuronin in their formulations by gradient elution within 15 and 20 min respectively. % RSD in retention times (R.T.) of Bispyribac sodium and Diafenthiuron was found to be 0.060 and 0.218 respectively. % RSD in the peak area of Bispyribac sodium and Diafenthiuron was found to be 0.218 and 0.403. The % recovery of Bispyribac sodium and Difenthiuron was found to be 101.14 and 99.38%. Conclusion: The UFLC method for assay of Bispyribac sodium and Difenthiuron was successfully developed, validated, and demonstrated to be accurate, precise and specific.