Molecular characterization of pea (Pisum sativum) genotypes by utilization of RAPD markers

A Random Amplified Polymorphic DNA (RAPD) marker was used for identifying
and mapping the population in pea (Pisum sativum). The presence of multiple polymorphisms
between cultivars and lines revealed at least one fragment for any given primer was present in
the DNA of one form of pea and absent in the DNA of another line or cultivar. Polymerase
chain reaction (PCR) based molecular marker viz. random amplified polymorphic DNA was
applied to 20 germplasm of Pea to assess the degree of polymorphism within the genes and to
investigate the genetic studies in Pea. This study, using 20 germplasm of pea was evaluated
for variability using a panel of 14 random l0-mer primers. The polymorphisms in PCR
amplification products were subjected to the unweighted pair group method for arithmetic
averages (UPGMA) and plotted in a dendrogram based on similarity data showing that all the
cultivars analyzed were related. Eleven out of 14 primers revealed scorable 60-polymorphic
bands between cultivars of Pisum sativum and the rest did not show polymorphism in their
genetic level. All the 60 amplified bands were polymorphic and the numbers of bands
produced per primer ranged from band 3 to 11 bands. PIC, EMR, and MI values ranged from
0.22 to 0.37, 1.00 to 5.20, and 0.34 to 1.92 with the average of PIC, EMR, and MI values
being 0.34, 2.86, and 0.95 respectively. In addition, the value of resolving power (RP) ranged
from 0.80 to 6.20 with an average value of 2.59. GS (Genetic similarity) value ranged from
0.13 between genotypes VL-3 and Arka Ajit and 0.90 between genotype AP-3 and Arka
Priya.