SDS-PAGE profiling of dormant lines in mung bean (Vigna radiata L. Wilczek)

Mung bean is an important pulse crop because of its high protein content. But it is very much susceptible to in-situ germination. An attempt was made to develop such lines which are resistant to in-situ germination by crossing a wild form of mung bean with a cultivated variety and a total seed protein profiling for such lines along with parents was done. The SDS-PAGE of 20 mung bean dormant lines was done. It includes one derivative of the four-way cross, thirteen derivatives of three-way crosses, five cultivated varieties and one wild form of mung bean i.e. BWM-29 (progenitor of cultivated mung bean). The study revealed 10-11 detectable peptides whose molecular weight was found to be in the range of 19 kDa to 117 kDa. The total seed protein was isolated using TCA-Acetone buffer and separated on 10% SDS-PAGE gel. The scoring of resolved proteins was done and a zygogram was generated. The zygogram revealed three genetic clusters. The first cluster contains nine genotypes and the second and third clusters group four and six genotypes respectively. Cluster I consist of 13 breeding lines and Cluster II consists of five breeding lines. Cluster III and Cluster IV are solitarily consisting of one genotype each. The wild form (BWM-29) was clearly isolated into a single cluster. The derivative of the four way cross SPS-10 was also separated into a single cluster. Further, it was also revealed that breeding lines in the same cluster based on total seed protein profiling have common agronomic characteristics and such clusters with unique morphological and yield-associated traits could be identified for genetic improvement.