The groundnut crop is affected by various diseases caused by fungi, bacteria and viruses, among these diseases, stem rot is the most predominant, devastating as well as yield reducing factor of groundnut. In Telangana state, Khammam district is one of the important groundnut cultivating areas growing different varieties. In the recent past, the incidence of stem rot has increased at an alarm level. The survey would ultimately indicate the areas with high disease incidence which can be managed timely to avoid any disease epidemic. Keeping because of the importance of the disease, a roving survey was conducted and a collection of 15 isolates was done during October- December 2022 in the groundnut growing areas of erstwhile Khammam district. The study revealed variations in per cent disease incidence in major groundnut growing areas of erstwhile Khammam district. The per cent disease incidence was recorded highest in Thimminenipalem village of chintakani mandal (52.0) followed by keshavapuram village of kusumanchi mandal (45.0) of Khammam area. A minimum per cent disease Incidence was observed in Dhammapet village of Dhammapeta mandal, Khammam district (7%). The results have also shown that among the 15 isolates collected from the different groundnut cultivars grown in the mentioned groundnut growing areas shown variations in the per cent disease incidence in the decreasing order of SR12 (52.00), SR 13 (46.00), SR7 (45.00), SR11 (44.10), SR8 (42.02), SR15, (41.00), SR9 (36.00), SR3 (33.00), SR14 (32.34), SR4 (32.30), SR2 (28.00), SR10 (28.00), SR6 (23.53), SR5 (17.01) and SR1 (7.10), respectively.