Strawberry is the well-known crop in Dehradun (Uttarakhand) and is highly appreciated by the consumers in this region. The problem is to get qualitative yield. The present study was conducted to improve quality of the fruits regarding this an experiment was conducted at the main experiment station, DCAST, Dehradun during the years 2018-19 and 2019-20 for the optimization of physico-chemical characteristics of strawberry fruit through organic manure and inorganic fertilizers with the aid of bio-fertilizers. The experiment was statistically conducted in a completely Randomized Block Design (CRBD) with three replications having nine treatments with different doses of NPK, FYM, Azotobactor and PSB either single or in combination.The data noted for different physico-chemical characters vizFruit size, Weight of berry and Volume of berryTSS, Acidity, Ascorbic acid, Reducing sugar, Non-reducing sugars, Total sugars, and Anthocyanin were found significantly superior with soil application of T₅– 100% FYM+NPK (25:120:80) kg/hac+Azotobacter (20kg/ha)+PSB (20kg/ha) during both the year of experimentation 2018-19 and 2019-20 whereas all the treatment were noted better result for the different characters while the treatment T₁-50 ton FYM/ha was noted lowest result among all other treatments.