Seed vigour analysis using oxygen sensing technology in Rice (Oryza sativa. L) Genotypes

Seed quality test is most important in agriculture to determine the planting value and to
minimize the hazards of crop production. Quick and reliable vigour tests are required for
supply of quality seed to the farmer. However, most commonly used method for the
evaluation of vigour i.e. germination test is time-consuming and laborious. To rapidly assess
the seed vigour, a Q2 scanner based on Oxygen sensing technology is used. The conventional
vigour testing based on germination and seedling parameters needs a minimum of eight days
for assessment. On the other hand, Q2 analysis provides a fast and accurate single-seed
measurement of oxygen consumption during germination. Oxygen measurement can be used
as it is directly related to seed respiration and energy production. An analysis can take 10 to
72 hours, depending on the species. The result is an easy determination of the metabolic
activity of seeds. Although the Q2 analysis does not provide specific information about
seedling categories in terms of the International Seed Testing Association (ISTA) (normal,
abnormal etc.), it delivers fast and accurate information about homogeneity of seed vigour
linked to oxygen consumption of seed lots. The Q2 analysis can be used to determine
differences between seed lots in comparative trials. Oxygen-sensing technology provides a
fast and automatic measurement of oxygen consumption and respiration efficiency. The
present study is conducted for the evaluation of six rice genotypes for vigour using Q2
Scanner and examined the correlations between the different potential vigour indices.