The plants face so much stress which includes the biotic as well as abiotic. Abiotic stress like cold, drought, and heat stress is very common and these all are impacted the plants. The study of drought stress is the main and important component in improving plant health. The present study deals with the study of drought tolerance with the response of mycorrhiza on the rice plant. Drought tolerance is the mechanism in plants where the plant tolerates stress by producing antioxidants and develops some morphological changes. In this study, the rice plants were subjected to drought conditions, and various biochemical parameters were figured out from this research. The biochemical parameters like proline content where the AMF was more effective at the tillering phase of the crop rather than the reproductive phase of the crop. The antioxidants’ activity like catalase and SOD were shown high content with the inoculation of AMF in stress conditions. Total soluble sugar, lipid peroxidation, and ascorbate acid content showed positive results with the symbiosis of AMF in the stress condition. Therefore this study supports the research of inoculation of AMF in rice in drought stress but using two different varieties of rice.