Tag: Green synthesis

Green Synthesis of Zinc Oxide Nanoparticles with Citrus Peel

admin May 5, 2025

HTML Full Text PDF XML DOI: https://doi.org/10.21276/AATCCReview.2025.13.02.226 Abstract The synthesis of metal oxide nanoparticles with the use of plant extracts is a promising alternative to

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DOI: https://doi.org/10.21276/AATCCReview.2025.13.02.226

Abstract

The synthesis of metal oxide nanoparticles with the use of plant extracts is a promising
alternative to the conventional chemical method. This study aims to synthesize green zinc oxide
nanoparticles with zinc acetate dihydrate precursor using Citrus reticulata peel powder. The
synthesized nanoparticles were characterized for different analytic techniques. The maximum
UV-Vis absorption peak was observed at 357 nm wavelengths which is specific for zinc oxide
nanoparticles with a greater particle size of 36 nm. The FT-IR result shows a strongest
absorption bands at 651 cm -1 , which resulted from a covalent bond between zinc metal and
oxygen atoms (Zn-O). According to SEM analysis, the morphology of the biosynthesized ZnO
NPs were predominantly spherical in shape. Furthermore, the purity of the ZnO NPs was also
confirmed by EDS analysis. The crystallinity of the biosynthesized ZnO NPs was confirmed by
XRD analysis, revealing sharp and narrow diffraction peaks, confirming the hexagonal wurtzite
structure of the ZnO NPs.

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Green Synthesis of Silver Nanoparticles and Assessment of their In-Vitro Antibacterial Potential Against Common Pathogens in Bovine Semen

admin February 11, 2025

HTML Full Text PDF XML DOI: https://doi.org/10.21276/AATCCReview.2025.13.01.286 Abstract Bacterial contamination in bovine semen significantly impacts fertility and overall health in the cattle industry. Traditional antibiotics

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DOI: https://doi.org/10.21276/AATCCReview.2025.13.01.286

Abstract

Bacterial contamination in bovine semen significantly impacts fertility and overall
health in the cattle industry. Traditional antibiotics used to control this contamination
raise concerns about antibiotic resistance and potential adverse effects on sperm
quality. This study explores the use of green-synthesized silver nanoparticles (AgNPs)
as an alternative antibacterial agent for semen preservation. AgNPs were synthesized
using ethanolic extracts of Withania somnifera (ashwagandha) and Syzygium
aromaticum (clove) and their antibacterial efficacy was evaluated against common
semen pathogens. The synthesized AgNPs displayed superior antibacterial activity,
particularly those derived from ethanolic extracts, which were further characterized
for their size, shape, stability, and crystalline structure. UV-Vis spectroscopy, Zeta
potential measurements, TEM, XRD, and FTIR analyses confirmed the formation of
stable, spherical AgNPs with desirable properties for application in semen extenders.
This study highlights the potential of green-synthesized AgNPs as a sustainable and
effective alternative to antibiotics for reducing bacterial contamination in
cryopreserved semen, mitigating the risk of antibiotic resistance.
One significant challenge of the study is the variability in the synthesis and
characterization of AgNPs, which may affect their antibacterial efficacy and stability.
Additionally, the potential long-term effects of AgNPs on sperm quality and fertility
require thorough investigation to ensure their safety in practical applications.
Despite these challenges, the study contributes significantly to the cattle industry by
offering a sustainable and effective method to combat bacterial contamination in
cryopreserved semen. By demonstrating the superior antibacterial activity of AgNPs
synthesized from natural extracts, this research paves the way for reducing reliance on
traditional antibiotics, thereby addressing concerns related to antibiotic resistance and
enhancing overall reproductive health in cattle.

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